Optimization of Supercritical Fluid Extraction of Total Flavonoids and Monomer Compositions from Taxus Remainder Extracts Free of Taxoids

Objective: To optimize the supercritical fluid extraction technology of flavonoids from taxus remainder extracts free of taxoids( TREFT) by response surface methodology( RSM). Methods: By using a central composite design( CCD) in four factors and five levels to optimize the extraction parameters. The effects of extraction time, temperature, pressure and different concentration of ethanol and their interaction on the extraction rate of total flavonoids, amentoflavone, quercetin and ginkgetin which extracted from TREFT by supercritical fluid CO2 were investigated. Results: Under optimal conditions of extraction time for 2. 2 h,and the extraction temperature was 46. 4 ℃,the extraction pressure was 22. 6 MPa, and 80. 7% ethanol, the index experimental of total flavonoids and monomer compositions was 98. 75. Conclusion: The process of flavonoids extraction from TREFT is stable and feasible, which can provide technical support for the resource utilization of TREFT.