Immunogenicity and Specificity of Anti recombinant Protein Fim-C-Salmonella typhimurium Antibody as a Model to Develop Typhoid Vaccine☆

Abstract Salmonella typhimurium , that cause typhoid fever in mice was used as a model to study typhoid fever in humans. This study is aimed to determine the immunogenicity of recombinant protein of Fim-C- S. typhimurium and specificity of antibody anti-recombinant protein of Fim-C- S. typhimurium in ddY mice. The immunogenicity studies by ELISA indicated that recombinant protein of Fim-C- S. typhimurium evokes strong immune response in ddY mice, particularly in experiment group. The specificity evaluated by Western Immunobloting technique indicated that the antibody anti-recombinant protein of Fim-C- S. typhimurium can detect the 31 kDa of recombinant protein of Fim-C- S. typhimurium as an antigen. This data reported that recombinant protein of Fim-C- S. typhimurium is immunogenic and the antibody anti-recombinant protein of Fim-C- S. typhimurium is specific. The results of this study can be used as a model to develop recombinant vaccine candidate against typhoid.