Identification of Clinical Isolates of α-Hemolytic Streptococci by 16S rRNA Gene Sequencing, Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using MALDI Biotyper, and Conventional Phenotypic Methods: a Comparison
2012
Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMerieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.
Keywords:
- Time-of-flight mass spectrometry
- Matrix-assisted laser desorption/ionization
- Microbiology
- DNA sequencing
- Ribosomal RNA
- Intergenic region
- Sequence analysis
- Streptococcus
- Biology
- Molecular biology
- 16S ribosomal RNA
- species level
- gene sequence
- Mass spectrometry
- 16s rrna gene sequencing
- matrix assisted laser desorption ionization time of flight
- Correction
- Source
- Cite
- Save
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