9-顺－维甲酸对肺癌细胞周期及周期因子表达的影响

Aim To study the effects of 9-cis-retinoic acid (9-cis-RA) on eel1 cycle and expression of cyclin D1 and cdk4 in lung cancer cells. Methods 9-cis-RA (1×10^(-6) mol•L^(-1)) was used to treat lung cancer cells for 24 h; Flow cytometry (FCM) was used to detect the percent of G0/G1 phase and S phase cells of three groups including blank control, DMS0 control and 9-cis-RA groups; RT-PCR was used to analyze the expression changes of cyclin D1 and cdk4 before and after treatment with 9-cis-RA in lung cancer cells. Results The percent of Go/G1 phase cells of 9-cis-RA groups was significantly higher than that of the control groups (P＜0.01 or P＜0.05) and the percent of S phase cells of 9-cis-RA groups was lower than that of the control groups (P＜0.01 or P＜0.05); the expression of cyclin Dl of PG. SPC-A1 and 128 cells was decreased (P＜0.01) and the expression of cdk4 of PG, A549 and L78 cells was also decreased (P ＜0.01) after treatment with 9-cis-RA. Conclusion Most of the proliferation and the expression of cyclin Dl and cdk4 of PG, A549, SPC-A1 and L78 were inhibited by 9-cis-RA.