Labeling of the Hydrophobic Core of Membranes with 3-trifluoromethyl-3-(m-[125I]iodophenyl) diazirine: Measurement of the Time-Course of the Photolabeling Process

Labeling of membranes from within the lipid core aims at identifying those polypeptide segments of membrane proteins that are buried within the lipid bilayer (Brunner 1981, Bayley 1982). This method, therefore, represents a useful complement to the more established surface labeling techniques of membranes.