Rapid PCR technique to detect QoI-resistant strains of Magnaporthe oryzae

The emergence of Magnaporthe oryzae strains with resistance to quinone outside inhibitor (QoI) fungicides necessitates the development of easy techniques for the rapid, sensitive monitoring of such strains. A QoI-resistant mutant and a wild-type strain showed a single nucleotide polymorphism (SNP). For simple, fast, low-cost, and reliable detection of this SNP, we developed two types of PCR-based DNA markers that enable easy detection of resistant strains by using DNA templates prepared from filter paper permeated with the fungus. The method enabled us to obtain reliable results by using a detection procedure that takes only a few hours without special kits.