RAPID EFFECT OF GLUCOCORTICOID ON EXCITATORY POSTSYNAPTIC POTENTIAL OF B CELLS IN BULLFROG PARAVERTEBRAL GANGLIA

In the present study, the rapid effect of hydrocortisone 21-hemisuccinate (F-suc) on the amplitude of f-EPSP of B cells in isolated bullfrog symathetic ganglia was examined with the use of intracellular recording technique. It was found tha the amplitude of f-EPSP of B cells was decreased by F-suc, with a latency less than 3 min after the beginning of perfusion of F-suc. The inhibition of F-suc was concentration-dependent and could be blocked by the antagonist of intracellular glucocorticoid receptor RU38486, but not by the protein synthesis inhibitor actidione. These findings suggest that the inhibition of f-EPSP of B cells by F-suc is probably mediated through nongenomic mechanism.