신규 Lactobacillus paracasei SKB1192의 피부 광노화억제 효과

The continuous exposure of ultraviolet-B (UVB) causes the induction of reactive oxygen species (ROS) resulting in an increase of matrix metalloproteinases (MMPs) and a decrease of procollagen in skin. Its deleterious phenomenon is called “skin photoaging”. To prevent skin photoaging, various antioxidants are used to remove ROS. However UVB cannot be blocked by antioxidants directly which can adversely affect the skin microbiome and skin. In this study, we isolated the strongest UVB absorbing strain among 17 latic acid bacteria, and identified Lactobacillus paracasei SKB1192 (GenBank Accession No. MK511782) from Jeju citrus traditional Makgeolli by 16S rRNA sequencing. Also, we examined the effects of L. paracasei SKB1192 on HaCaT cells. It reduced the UVB-induced inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) without cytotoxicity. By using JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine) assay, it was found that L. paracasei SKB1192 interfered 41.1% UVB transmission to HaCaT cells by direct absorbance of UVB, compared with control. In conclusion, we suggest that L. paracasei SKB1192 protects epidermis from UVB, and thus provides novel approach for prevention of skin photoaging.