Time-dependent classification of protein diffusion types: A statistical detection of mean-squared-displacement exponent transitions

In this paper, we have proposed a statistical procedure for detecting transitions of the mean-square-displacement exponent value within a single trajectory. With this procedure, we have identified three regimes of proteins dynamics on a cell membrane, namely, subdiffusion, free diffusion, and immobility. The fourth considered dynamics type, namely, superdiffusion was not detected. We show that the analyzed protein trajectories are not stationary and not ergodic. Moreover, classification of the dynamics type performed without prior detection of transitions may lead to the overestimation of the proportion of subdiffusive trajectories.