[On the effect of E. coli-12 treated with liquid-paraffin against the development of experimental murine leprosy in the mice (author's transl)].

The mice were divided into two groups, each group being composed of the control groups and the treated ones.Preparation of an oil-in-water-in-oil-in-water emulsion : 0.1mg. of E. Coli-12 cells was freezed and dried and 10% Emazol-liquid-paraffin was at once added to them until it was easily dispersed and penetrated thoroughy of itself in them and then the water was added as shown in Table 1 and Table 2. The above-made mixture was heated in warm water less than 50°C. and it was diluted 1500 fold or 4000 fold (referred to A-emulsion). The A-emulsion was kept at 4°C until use. It had a characteristic feature that it was never separated in each component, even when it was kept quiet. Preparation of a water-in-oil-in-waser emulsion: it was made and kept by means of the above-mentioned same process only excepting freezing and drying the E. Coli-12 cells. (referred to B-emulsion). Its striking characteristic was that the cells were more swollen and so the 10% Emazolparaffin was little dispersed and penetrated in them therefore, it was easily separated of itself in each component when it was kept quiet.The treated mice were previously given subcutaneous and intraperitoneal injections with 0.1mg. of the A or B-emulsion simultaneously twice a week for one month. One week later, all the animals were subcutaneouly injected with 0.1mg. of the bacillus. At the end of the experiment, all the survivors were killed for macroscopic and microscopic studies. Pathological appearance : Severe destructive leprous lesions were recognized in the control groups; the multiplication of histiocytes followed that of the bacilli, becoming a specific nodular mass of histiocytes and the formation of muri-leproma accompanied by slight perivascular infiltration of inflammatory cells and what was called by globi could be found in the control groups2), 3), 4), while in marked contrast was the regression of active lesions in the A-emulsion-treated groups; the formation of epithelioid granuloma sometimes containing Langhans type giant cells and including granular-shaped degenerative acid-fast bacilli could be observed, the inflammatory cells surrounding the granulomas being found2), 3), 4).It is concluded that the A-emulsion-treated groups revealed favourably more protective power against the development of the progressive leprosy here, as determined by the clinical, macroscopic and micoscopic examinations2), 3), 4).The principle included here can be applied not only in the treatment of the inflammatory diseases but also be probably on the high road of paving the way for the immunotherapy of the malignant tumors17), 18), 19), 20).