Inhibiting Effect of Dark Septate Endophyte Fungi D202 on Rhizoctonia solani and Its Physiological Characteristics

[Objective]The research aimed to evaluate the biocontrol ability and physiological characterisitics of endophyte strain D202. [Method]Dark septate endophyte fungi D202 was evaluated against R. solani in confront culture and through production of non-volatile inhibitors,and the physiological characteristics of D202 were researched by growth rate method. [Result]In the dual culture,D202 could inhibit the growth of R. solani effectively,the inhibition rate was 67. 25%,and relative inhibit rate was 3. 54. The non-volatile antibiotics inhibited the growth of R.solani effectively,and the inhibit rate was 72. 35%. Strain D202 could grow well in PDA media whose pH was 7. 0. The optimum carbon sources for D202 growing were glucose,and the optimum nitrogen sources were peptone,and the optimum temperature for growing was 25 ℃.[Conclusion]Endophyte D202 had the potential to develop biocontrol agents.