Development and Application of An Indirect ELISA for Detection of Anti-SeV Antibodies

Using the recombinant and purified F(S) protein as antigen expressed in E.coli M15 and the anti-serum to F(S) protein as antibody,an indirect ELISA was successfully developed to detect antibody against Sendai virus.The recombinant F(S) protein antigen showed no cross-reaction with the positive sera of other 4 kinds of viruses containing MHV,Reo-3,ECT and PVM in mouse;coeffi- cient of variability percent (C.V%) of intro-batch duplicativity test and inter-batch duplicativity test was less 7%.Result of compari- son was tested between rf-ELISA and ELISA kit from National Institute For the Control of Pharmaceutical and Biological Products (NICPBP) showed that 98% concordance was obtained.Therefore,this rf-ELISA based on recombinant F(S) protein antigen has good sensitivity and specificity,can provide a simple and rapid assay for the antibody of Sendai virus.