Isolation, characterization and extraction of mer gene of Hg2+ resisting strain D2

Abstract Mercury-resistant strain D 2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence, growth, minimal inhibitory concentrations (MICs) of metals, antibiotic susceptibility and mer gene were conducted. The strain D 2 can grow in the medium containing 60 mg/L mercury ion. It presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa , and exhibits high MIC values for heavy metals and a large spectrum antibiotics resistance. The mer RT gene sequence was amplified from chromosome. Strain D 2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.