High-performance liquid chromatographic determination of indole alkaloids in a suspension culture of Tabernaemontana div aricata

Abstract A method is reported for the determination of alkaloids in cell cultures of Tabernaemontana species. The alkaloids were isolated by means of C 8 pre-concentration columns. After recovery they were analysed on a Phenyl type of column using phosphate buffer (pH 3.9)-acetonitrile-2-methoxyethanol (80:15:5) as the mobile phase. Detection was effected at 275 and 313 nm and peak-height ratios were used for identification.