A study of Pt4+ -adsorption and its reduction by Bacillus megaterium D01

The properties of Pt 4+ adsorption and its reduction by Bacillus megaterium D01 were studied by means of ICP, anode stripping voltammetry, TEM, IR and XPS. The results of ICP analyses showed that the Pt 4+ adsorptive efficiency of the strain D01 was as high as 94.3% under the conditions of 100 mg Pt 4+ /L, 1 g biomass/L, pH 3.5 and at 30 ℃ for 24 h. Moreover, it was confirmed from anode stripping voltammetry that the strain D01 possessed a strong reducibility. The TEM analysis indicated that the strain D01 was able to adsorb and reduce Pt 4+ to Pt 0, small particles. The XPS result further supported the reduction of Pt 4+ to Pt 2+ , followed by the further recuction to Pt 0. The IR spectrum implied that D01 biomass adsorption of Pt 4+ may result in the complexation of the CO bond to the Pt species.