Size Exclusion of Associating Systems. II. A Model Describing the Hindered Release of Solute from the Stationary Phase

Abstract During a separation process of reversibly associating species (e.g. tensides, block copolymers etc.) in size exclusion chromatography (SEC), some amount of solute can be temporarily trapped in the gel phase. A model covering this effect, namely hindered release of a solute form the gel phase, has been developed. Results of numerical calculations have been compared with experimental data of micellizing block copolymers and the effect of trapping of the solute in the gel pores has been demonstrated.