Application of BD FACSAriaIII flow cytometer in the research

Objective: To provide a basis for proper use of BD FACSAriaIII flow cytometer by analyzing the related causes of its malfunction. Methods: Through study of using BD FACSAriaIII flow cytometer in core facilities of State Key Laboratory of Experimental Hematology in 2012-2013, frequently occurred malfunction, solved malfunction by manufacturer's engineers and their causes and solutions are summarized. Results: To provide guidance of using high throughput cell sorters and improve the stability of running the instrument. Conclusion: Proper use and good repair helps to guarantee the normal operation of BD FACSAriaIII flow cytometer.