Comparative study of bacterial translocation control with nitric oxide donors and COX2 inhibitor

Abstract Objective and design To evaluate the beneficial effects of exogenous NO and an inhibitor of the COX2, and their action levels in a model of SIRS/bacterial translocation (BT) induced by Zymosan A ® . Material and methods Ninety Wistar rats were submitted to different treatments, and after 12 h and 24 h they were anaesthetized in order to collect blood, mesenteric lymph nodes, and kidney for subsequent biochemical analyses and microbiological examinations. Treatments A nitric oxide donor, Molsidomine ® , was compared with a COX2 inhibitor, Celecoxib ® . Methods Zymosan A ® was administered to Wistar rats. The animals were divided into 6 groups: one group for survival study, Group (1) No manipulation (BASAL); Group (2) vehicle of Zymosan A ® given intraperitoneally (SHAM); Group I (control), with Zymosan A ® (0.6 g/kg) intraperitoneally; Group II (Molsidomine), with Molsidomine ® (4 mg/kg) through the penis dorsal vein, 30 min prior to administration of the Zy ® (0.6 g/kg); Group III (Celecoxib), with Celecoxib ® (400 mg/kg) orally through a stomach tube, 6 h prior to administration of the Zy (0.6 g/kg). Determinations The parameters survival, bacterial translocation, renal function, neutrophil accumulation, oxygen free radicals (OFR), detoxifying enzymes, and cytokines were measured at different times after Zymosan administration. Results The model established induced a mortality rate of 100% and generated BT and systemic inflammatory response syndrome (SIRS) in all samples. It also significantly increased all variables, with p p p ® showed a lower capacity for SIRS regulation. Conclusions The exogenous administration of NO prevented BT and controlled SIRS. Therefore these results suggest that Molsidomine could be used as a therapeutic strategy to protect against BT.