Adenosine and opiate-like substances mediates antinociception at the spinal cord.

Abstract The effects of intrathecally administered naloxone or aminophylline on the antinociception produced by intrathecal NE, 5-HT, morphine or adenosine receptor agonist, 5′- N -ethylcar☐amidoadenosine (NECA) were observed in rats using the tail-flick test. The results show that: (1) the antinociception produced by NE with doses of 0.5 or 1.0 nmol could be completely blocked by both naloxone (240 nmol) and aminophylline (120 nmol); (2) neither naloxone (240 nmol) nor aminophylline (120 nmol) could alter the antinociception produced by 5-HT with doses of 60 or 120 nmol; and (3) the antinociception produced by morphine (0.5 nmol) could be blocked by both naloxone (240 nmol) and aminophylline (120 nmol), while the antinociception by NECA (0.5 nmol) could be blocked only by aminophylline (120 nmol), but not by naloxone (240 nmol). The results suggest that opiate-like substances (OLS) and adenosine are involved in the mediation of the NE-produced antinociception, but not in 5-HT-produced antinociception. Results also suggest that NE, OLS and adenosine may act in a sequential order in the performance of NE-induced antinociception at the spinal level.