Purification and characterization of an ovulation stimulating substance from the male accessory glands of Drosophila suzukii

Abstract An ovulation stimulating substance (OSS) was isolated from males of the fruit fly Drosophila suzukii , and purified to a homogeneous state by a 5-step purification procedure: extraction with 80% methanol, chloroform wash, heat treatment, ion-exchange chromatography, and reverse phase high performance liquid chromatography. Approximately 100-fold purification was obtained thereby yielding 39 μg of OSS from 1000 males for an overall yield of 34%. The OSS is a single peptide consisting of at least 35 amino acid residues and having a molecular weight of 3990. The purified OSS not only initiated ovulation in unmated females but also suppressed their receptivity towards males. The peptide of D. suzukii was found to be effective in the females of D. melanogaster , a species that belong to a different subgroup, but was less effective in a more closely related species, D. pulchrella .