Establishment of a quick and facilitate approach to harvest sweat gland cells in vitro

Objective To establish a rapid approach of isolating sweat gland cells （SGCs） compared with the traditional method in vitro. Methods Through diminishing particles of the skin （ diameter was reduced to 0. 5-1.0 mm） , elevating the saturation of the collagenase type Ⅱ（2. 5 g/L） , enhancing the environmental temperature （37.5℃）, the time of harvesting SGCs, the morphology, outgrowth ability, and the proliferation of SGCs obtained by this method were compared with the previous method of obtaining SGCs. Results Compared to previous method, the time of harvesting SGCs by this approach was short- ened obviously from more than 12 h to 3-5 h, meanwhile there was no significant difference in morphology and proliferation ability between them （P 〉 0. 05 ）. Conclusion A rapid and promising method for isolating SGCs in vitrowas established, which supply a new approach of harvesting cells for the study of repair and regeneration of sweat glands. Key words: Sweat gland cells ;  Isolation ;  Culture;  Skin regeneration