Effects of Dokhwalgisaengtang-gami Water Extract on Osteoclast Differentiation and Osteoblast Function in RANKL-induced RAW 264.7 Cell

ABSTRACT Effects of Dokhwalgisaengtang-gami Water Extract on Osteoclast Differentiation and Osteoblast Function in RANKL-induced RAW 264.7 CellYun-Mo Je, Dong-Youl YooDept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon UniversityObjectives: This study was performed to evaluate the effect of Dokhwalgisaengtang -gami water extract(DGG) on osteoporosis. Methods: The osteoclastogenesis and gene expression were determined in RANKL -stimulated RAW 264.7 cell. And osteoblastogenesis was also determined in rat calvarial cell.Results: The results were summarized as followes.1. DGG decreased the number of TRAP positive cell in RANKL-stim ulated RAW 264.7 cell.2. DGG inhibited TRAP activity in RANKL-stimulated RAW 264.7 cell.3. DGG decreased the expression of NAFTc1, MITF in RANKL-stimul ated RAW 264.7 cell.4. DGG increased the expression of iNOS, COX-2, IL-6 in RANKL-s timulated RAW 264.7 cell.5. DGG decreased the expression of cathepsin K, MMP-9, TRAP in RANKL -stimulated RAW 264.7 cell.6. DGG increased cell proliferation of rat calvarial cell.7. DGG increased ALP activity in rat calvarial cell8. DGG increased bone matrix protein, collagen synthesis and no dule formation in rat calvarial cell.Conclusions: It is concluded that DGG might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's r elated gene expression. And DGG might increase the bone formation resulted from increase of osteoblast function.Key Words: Dokhwalgisaengtang-gami(DGG), Osteoporosis, Osteoclastogenesis, Osteoblastogenesis, Osteoclast, Osteoblast