The identification of Long non-coding RNA H19 target and its function in Chronic myeloid leukemia (CML)

Abstract: H19 is a Long non-coding RNA which was lowly expressed in CML. Here，we found that the overexpression of H19 significantly inhibited cell viability and colony formation, and prolong survival in CML cell lines and three xenografted mouse models. The H19 target proteins and miRNAs were identified using a combination of computational prediction and RNA-Pull down, including PCBP1, FUS protein and miR-19a-3p, miR-106b-5p. Targeting PCBP1, FUS protein, miR-19a-3p and miR-106b-5p significantly inhibit the cell growth and colony formation of CML cell lines. Co-overexpression of H19 and PCBP1, FUS, miR-19a-3p and miR-106b-5p decrease the inhibitory effect of H19 in CML. These findings might provide a novel molecular insight into CML.