Facile and high selective ratiometric fluorescence probe based on benzo[5]helicene for the detection of hypochlorous acid

In physiological processes, hypochlorous acid can regulate vitro signal molecules as one kind of reactive oxygen species (ROS). Serious diseases, such as cardiovascular disorders, neurodegenerative diseases, Alzheimer’s disease and cancers are usually concerned with the abnormal levels of the hypochlorous acid in human bodies. Herein, we report a new probe DPH-BP for HClO, which consists of a benzo[5]helicene-pyridine fluorophore and benzyl-benzeneboronic acid pinacol ester as HClO reaction sites. The structural motifs combined with chromogenic and fluorescence becomes a single molecule sensor response in aqueous solutions. In the presence of HClO, this probe shows a remarkable blue shift of fluorescence from 536 nm to 452 nm in aqueous solution and obvious fluorescence color change from yellow to blue. In the same conditions, almost no response can be recorded with the other common ROS/RNS, such as H2O2, TBHP, •OH, t-BuO•, ONOO-, and O2•-. As we have designed, the oxidation of phenyboronic ester by HClO ...