Reproductive responses following royal jelly treatment administered orally or intramuscularly into progesterone-treated Awassi ewes

Abstract An experiment was conducted to determine whether natural royal jelly (RJ) paste administered orally or intramuscularly (i.m.) in conjunction with exogenous progesterone is associated with improved reproductive responses in ewes. Thirty 3–6-year-old Awassi ewes were randomly allocated into three (RJ-capsule, RJC; RJ-injection, RJI and control, CON) groups of 10 ewes each. All ewes were treated with intravaginal progesterone sponges for 12 days. Ewes in the RJC and RJI were administered orally or i.m. with a total of 3 g of RJ given in 12 equal doses of 250 mg per ewe per day starting at the time of sponge insertion. At the time of sponge withdrawal (day 0, 0 h), ewes were exposed to three rams and checked for breeding marks at 6-h intervals for 3 days. Blood samples were collected from all ewes for analysis of progesterone concentrations. Pretreatment progesterone levels were −1 in 16/30 and >1.3 ng ml −1 in the remaining ewes indicating luteal function and cyclicity. Similar reproductive responses and progesterone levels occurred in ewes of the RJC and RJI; therefore, data of the two groups were pooled. Following sponge insertion, progesterone levels increased rapidly and reached maximum values of 5.8±0.2 ng ml −1 within 2 days among ewes of the three groups, and then declined gradually to day 0 values of 1.6±0.1 and 1.9±0.1 ng ml −1 for the RJ-treated and CON ewes, respectively. The rate of progesterone decline was greater ( P P −1 versus 3.3±0.2 ng ml −1 ). Treatment with RJ resulted in greater ( P P P =0.09). All pregnant ewes exhibited oestrus 14 h earlier ( P P P