Developing methods to evaluate phenotypic variability in biological nitrification inhibition (BNI) capacity of Brachiaria grasses

O emissions. The current methodologies for quantifying the BNI trait need enhancement to accelerate the process of identify-ing differences between genotypes. In this paper, we aim to develop new (or improve the existing) phenotyping methods for this trait. Preliminary results were obtained using 3 different methods to quan-tify BNI: (1) a mass spectrometry method to quantify brachialactone; (2) a static chamber method to quantify N