Inhibiting the leaching of lipase from mesoporous supports by polymerization of grafted vinyl groups
2006
Abstract This communication reports a method to prevent leaching of porcine pancreatic lipase (PPL) from the pore channels of SBA-15, whereby the pore size is reduced by chemical modification after uptake of porcine pancreatic lipase (PPL). The concrete procedure involves silylation of the surface of SBA-15 entrapping PPL with 3-(trimethoxysilyl)-propylmethacrylate (H 2 C C(CH 3 )CO 2 (CH 2 ) 3 Si(OCH 3 ) 3 , PMA) followed by polymerization of the pendant vinyl groups and free PMA, initiated by 2,2′-azobisisobutyronitrile (AIBN).
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