A Study of Tumor Apoptosis Induced by Medicine Serum of Traditional Chinese Medicine

Objective Adopting serological methods of compound Chinese medicine that including 'Yi-Fei-Yin' to treat lung cancer, 'Yi-Chang-Tai' to treat lung cancer and 'Wei-Ai-Ning' to treat gastric cancer to explore the induced effect on the tumor cell apoptosis. Methods (1) Culture conventionally A549 human lung cancer cell lines, CCL229 human colorectal carcinoma cell lines and gastric cancer cell line SGC7901, and observe under the inverted microscope. (2) Three Chinese medicine compound which were decoction, three consecutive days of intragastric administration to rats, last administration was two hours before the animals were killed, according to the serum drug Preparation prepared medicinal serum. (3) Three drugs were cultured in serum of A549 human lung cancer cell lines, CCL229 human colorectal carcinoma cell lines and cell line SGC7901 48 hours. (4) With rubber scraper scraped cells, cell suspension will be transferred to the centrifuge tube centrifuge 2000rpm/min, 15min, Agar processing into pieces, washed with PBS, 1% osmium acid fixed, gradient ethanol and acetone dehydration, epoxy-embedded mesh, the conventional ultra thin section, electron stain. (5) To observe and take a picture under the JEM1200 TEM. Results (1) The parapodium of tumor cells decreased even disappeared. endocytoplasmic reticulum, mitochondria and other cell organdies for Poly IC, chromatin condensation, margination a 'crescent'. (2) It is apoptosis characteristic structure-the membrane parcels, containing some of the broken device and cell chromatin of apoptotic bodies. Conclusion Compound Chinese medicine serum has the function to induce tumor cells apoptosis, widely used for clinical medicine compound against lung cancer, gastric cancer and colorectal cancer provide reliable experimental evidence.