Wide-field Two Photon Microscopy

Two Photon Microscopy (2PM) generates microscopic images out of depth of biological samples. Up to now the method is restricted by narrow limitations of the field of view, the imaging depth and the orientation of the image field. A new approach overcomes these boundaries and delivers high resolution images revealing very specific information on clinically and biologically relevant tissue and cell structures. The status of the 2PM technology is critically reviewed and the options are discussed. The advantages of the new approach demonstrated by excellent tissue images.