Genetic polymorphism of D10S2325 locus and certifcation of rare alleles

Objective To identify the genetic polymorphism of D10S2325 locus in Fujian population and certify the rare alleles in order to evaluate for potential in forensic application. Methods 12 STR loci were amplified in DNA samples from 200 unrelated individuals in Fujian population using Investigator HDplex system. Gene frequencies and genetic parameters of D10S2325 locus were obtained. Primers of D10S2325 locus were designed and rare alleles were proved by sequencing analysis. Results 17 alleles of D10S2325 locus were found in Fujian population and gene frequencies of alleles were from 0.0025 to 0.1475. The personal identification rate (DP) was 0.9498, the probability of exclusion (PE) was 0.7415, and the polymorphism information content (PIC) was 0.8276. Sequencing analysis showed that the rare allele 24 of D10S2325 locus was [TCTTA]24. Conclusion D10S2325 locus can be used as a genetic marker because of the high polymorphism in Fujian population. In practice, the rare alleles of D10S2325 locus need to be accurately identified when investigator HDplex system has been used. Key words: STR locus; Polymorphism; Rare allele