Macro fungus demethylation breading method and macro fungus demethylation breading device

Disclosed are a macro fungus demethylation breading method and a macro fungus demethylation breading device. The device comprises a culture dish body and a culture dish cover, the culture dish body comprises a bottom disc and a side wall, and at least one annular partition is arranged in the culture dish body. The central area of the culture dish body is a pure culture medium area, and a culture medium A is added in the pure culture medium area; a culture medium A and an inhibitor B are added in mutation areas adjacent to the central area and continuous other mutation areas of the culture dish body; bacterial strains to be mutated are inoculated in the pure culture medium area, and are subjected to inverted culture under suitable-for-growth conditions; mycelia of the bacterial strains to be mutated in the central area grow into the adjacent mutation areas, and the inhibitor enters the growing mycelia to militate; mutant strains are acquired. The macro fungus demethylation breading method and the macro fungus demethylation breading device have the advantages that the device is simple in structure and convenient to use; the method is simple, mutation and detection can be performed synchronously, and mutation can be performed on macro fungi quickly and efficiently.