Quantitative determination of E5880 in rat plasma by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
1999
A simple and sensitive method is described for the determination of E5880 in rat plasma. The method is based on high-performance liquid chromatography/electrospray ionization mass spectrometry, using deuterated E5880 as an internal standard. Selected reaction monitoring is employed for selectivity and sensitivity, this in turn enables quantification in a short period of time (within 7 minutes) over the extended range of 0.1-1000 ng/ml with acceptable precision and accuracy. The method demonstrated to be suitable for the quantitative analysis of E5880 in rat plasma. The pharmacokinetic profile of E5880 after a single intravenous administration of E5880 was elucidated.
Keywords:
- Selected reaction monitoring
- Analytical chemistry
- Sample preparation in mass spectrometry
- Direct electron ionization liquid chromatography–mass spectrometry interface
- Tandem mass spectrometry
- Electrospray ionization
- Quantitative analysis (chemistry)
- High-performance liquid chromatography
- Chromatography
- Chemistry
- Mass spectrometry
- Electrospray
- Correction
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