Embryogenesis and plant regeneration of Clematis 'Multi-Blue'

Using stem tip of Clematis 'Multi-Blue' as explants and 1/2 MS as culture media,the suitable conditions for induction of embryogenic callus and somatic embryos,as well as plant regeneration,were determined.The results indicated that the optimal conditions for induction of embryogenic callus were 1/2 MS supplemented with 1.0 mg/L TDZ,0.5 mg/L NAA and 2 % scourse plus 0.7 % agar.Explants cultured in this solid medium could form yellow massive or granular callus and produced embryogenic callus after subculture.Suitable media for somatic embryo induction were 1/2 MS added with 0.2 mg/L TDZ and 0.1 mg/L IBA plus 0.5 g/L CH,with an induction rate of 96%.The germination rate of somatic embryos was the highest when the plant growth regulators were 0.2 mg/L 6-BA and 0.05 mg/L IBA.The plantlets with root were successfully transplanted to the medium with vermiculite.The survival rate of regeneration plant could be up to at least 80 %.