Significance of pH for the growth of the sisomicin producer and biosynthesis of the antibiotic

: Dependence of the mycelium growth and sisomycin production on the pH values of the fermentation broth was studied in cultures of various ages for 6 hours with the method of pH control within the preset levels. It was shown that the values of pH within the range of 7.25 - 7.6 were optimal. The pH values of 7.5 - 8.0 and 7.0 - 8.0 were optimal for the growth of the 24-hour culture and biosynthesis of the antibiotic by it respectively. pH 7.5 was optimal for the growth of the 48-hour mycelium and biosynthesis of the antibiotic by it.