Cytotoxic effect of anti-H-2 and anti-Ia antisera on human B and T cells.

Anti-H-2 and anti-Ia alloimmune mouse antisera were tested by the microcytotoxicity test on human peripheral blood B- and T-cell preparations. Anti-H-2 antisera react by a higher titer and/or cytotoxicity scoring grade on B cells than on T cells. Anti-Ia antisera react practically only with B cells. It was assumed that anti-H-2 antisera contain two components. One component reacts specifically with certain HLA-A-or -B-locus antigens or other closely linked gene products. The other component reacts predominantly or only with B-cell determinants; the specificity of the latter component has not yet been studied in sufficient detail.