Spatially-Engineered Janus Hybrid Nanozyme Toward SERS Liquid Biopsy at Nano/Micro Scales

Nanomaterials with intrinsic enzyme-mimicking properties (nanozymes), have been widely considered as artificial enzymes in biomedicine. However, manipulating inorganic nanozyme into multivariant targeted bio-analysis is still challenging because of the insufficient catalytic efficiency and biological blocking effect. Here, we rationally designed a spatially-engineered hollow Janus hybrid nanozyme vector (h-JHNzyme) based on the bifacial modulation of Ag-Au nanocage. The silver face inside the h-JHNzyme serves as an interior gate to promote the enzymatic activity of the Ag-Au nanozyme, while two-dimensional DNAzyme-motif nanobrushes deposited on the exterior surface of the h-JHNzyme endowed the targeting function and tremendously enhanced the peroxidase-mimicking activity. We demonstrated that the spatially-separated modulation of the h-JHNzyme propels it as powerful “all-in-one” enzymatic vector with excellent biocompatibility, specific vectorization, remarkable enzymatic performance and clinical practica...