Phytochemical screening and potential anticonvulsant activity of aqueous root extract of Decalepis nervosa Wight & Arn.;(TJPS-2021-0080.R3)

The effect of phytochemicals in the aqueous root extracts of Decalepis nervosa Wight & Arn. (ARDN), (Family: Apocynaceae), was investigated on an anticonvulsant action in laboratory animals (mice). The DN roots were extracted using microwave extraction method with aqueous solvent using two parameters, such as temperature variation and extraction time. The phytochemical screening followed by TLC and HPTLC studies was performed. The pentylenetetrazole and isoniazid models were used with two doses, 250 mg and 500 mg, for the anticonvulsant effects of the ARDN extract. The microwave extraction during 15 minute at 60oC temperature revealed the DN root aqueous extract yielded higher (36.20%) than other selected parameters. The phytochemical screening study indicated the existence of alkaloids, flavonoids, glycosides and phenols and further TLC, HPTLC studies confirmed the presence of Quercetin and Gallic acid that demonstrated a strong dose dependent anticonvulsant activity. The other biochemical parameters such as lipid peroxidation level showed significant dose dependent inhibition in malondialdehyde (MDA) content (29.86%) in mice brain and also glutathione content showed significant dose dependent increased in percentage content (58.22%) in brain of mice. Finally, ARDN’s potential anticonvulsant was first established through the optimized microwave extraction method with the presence of polyphenoilc phytoconstituents.