Mechanism of microRNA-30b in acute myocardial ischemia of mice

Objective Through the establishment of an animal model of acute myocardial ischemia in mice, to investigate the changes of plasminogen activator inhibitor-1 (PAI-1) and the action mechanism of microRNA (miRNA, miR)-30b during acute myocardial ischemia. Methods Mice were divided into the model group and the control group. Acute myocardial ischemia animal model was established after occlusion of left anterior descending coronary artery in the model group. The changes of PAI-1 and miR-30b in the myocardium and blood of mice were detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR), Western blotting, and enzyme linked immunosorbent assay (ELISA). Results The mRNA and protein expression levels of PAI-1 in the myocardium and blood of the model group were significantly up-regulated, and the miR-30b expression was significantly down-regulated, with statistically significant differences (t=1.031, -4.573, -1.968, P<0.05). The dual luciferase gene report indicated that PAI-1 was a direct target gene of miR-30b. Conclusion The expression of PAI-1 in the model of the acute myocardial ischemia was significantly up-regulated and hyperfibrinolysis occurred, which may be related to the down-regulation of miR-30b. The miR-30b may be involved in the process of acute myocardial ischemia through the regulation of PAI-1. Key words: MicroRNA-30b; Acute myocardial ischemia; Plasminogen activator inhibitor-1