Effects of 17β-estradiol on Vitellogenin Inducement and Gonad Development in Male Tanichthys albonubes
2008
Vitellogenin (Vtg) was purified from whole body
homogenate (WBH) of estradiol-17β (E2) treated male Tanichthys
albonubes by gel filtration (Sephacryl S-300). The results showed
that the Vtg appeared to exist as homotrimer of approximately 440 kD
through native polyacrylamide gel electrophoresis (PAGE). Vtg was
characterized as a phospholipoglycoprotein by native-PAGE and staining
of gels for CBB R-250, for carbohydrates with periodic acid-schiff
reagent, for phosphorus with methyl green and for lipids with sudan
black B. Body weight decreased (P<0.01) and the development of sperm
was delayed after E2 treatment when compared with the control group of
male fish. The results indicated that E2 can induce male fish to
produce large quantities of Vtg and have a negative effect on its
growth and gonad development. It also indicated that Vtg of the male T.
albonubes could be as a valid biomarker for environmental estrogens
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