Direct organogenesis from leaf explants of Garcinia indica Choisy: An important medicinal plant

A high frequency plant regeneration protocol through direct organogenesis has been developed for Garcinia indica Choisy using leaf explants. The leaf explants were procured from the in vitro developed shoots raised from the nodal explants comprising of axillary buds. The leaf explants were cultured on MS medium supplemented with different concentrations of BAP. The highest number of shoots per explant was obtained on the medium supplemented with 22.2 µM BAP, while the lowest number of shoots was recorded on the medium supplemented with 0.45 µM BAP. The highest percentage of rooted plants was obtained on MS basal medium (MS +0.45 µM BAP) supplemented with 0.81-1.07 µM NAA, while maximum root length was recorded on the medium supplemented with 0.05 µM NAA. MS medium supplemented with 0.45 µM BAP and 0.81 µM NAA was recorded optimal for rooting of the shoots. The plantlets were hardened and successfully acclimatized to the open field conditions.