SHORT COMMUNICATION Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

ophenol solution at 30 C for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) -1 -1 medium supplemented with cycloheximide (50 µg mL ) and nystatin (50 µg mL ). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.