Iodine-azide reagent in detection of thiophosphoryl nucleotides in thin-layer chromatography systems

Abstract The TLC detection limits of the phosphorothioate analogs of nucleotides and related compounds by means of UV, iodine, HCl vapours, the iodine–azide reagent and the molybdate reagent have been determined. The iodine–azide reagent has been applied for the selective TLC detection of the phosphorothioate analogs of nucleotides.