Экспериментальный инфаркт миокарда у крыс: особенности моделирования и течения в первые 48 часов после лигирования коронарной артерии

Objective: to identify the features of modeling and give a morphofunctional assessment of the course of experimental myocardial infarction (EMI) in rats within the first 48 hours after ligation of the left coronary artery (LCA). Material and methods. EMI was reproduced according to the method of H. Selye [1] in Jian Ye`s modification [2] by means of LCA ligation in 24 Wistar rats. Electrocardiographical, histological, biochemical studies were performed. The control group consisted of 7 falsely operated animals in which the chest had been opened without manipulation on the LCA. Results. 5-15 minutes after LCA ligation, an ischemic change in the (Q)RST complex was observed as a monophasic curve. The Q wave appeared on the EGG after 1 hour of EMI in 20% cases, after 7 hours in 88% cases, in 100% cases after 27 hours and remained by 48 hours of EMI. Histologically, within the first 7 hours there was acute alterative myocardial damage followed by a quantitative increase in diffusely located necrotic cardiomyocytes (CMC) by 27 hours and the appearance of a clear zone of coagulation necrosis by 48 hours; which was accompanied by corresponding changes in the level of troponin I. Conclusion. The course of EIM after LCA ligation is characterized by the staging of myocardial changes similar to that in humans, which provides a prerequisite for possible extrapolation of the results of the studies to humans.