Suppressive effect of Salvia miltiorrhiza extract on extracellular release of HMGB1.

Objective:To study the effects of Salvia miltiorrhiza on the release of high mobility group box 1(HMGB1) from endotoxin-stimulated macrophages and serum HMGB1 level of endotoxemia mice.Methods:HMGB1 concentration in the culture medium and expression of HMGB1 mRNA were analysed by enzyme-linked immunosorbent assay and RT-PCR,respectively,after 100 ng/ml lipopolysaccharide(LPS)-stimulated macrophage-like RAW 264.7 cells were treated with different doses of Salvia miltiorrhiza extract.In addition,the effect of Salvia miltiorrhiza extract was investigated on serum HMGB1 level and survival rate of endotoxemia mice which were established by intraperitoneal injection of LPS. Results:Salvia miltiorrhiza extract markedly suppressed the release of HMGB1 and expression of HMGB1 mRNA in LPS-stimulated macrophages,and decreased serum HMGB1 level of endotoxemia mice with the survival rate elevated. Conclusion:Salvia miltiorrhiza suppressed extracellular release of HMGB1 in endotoxin-stimulated macrophages,and conferred protection against endotoxin lethality.