Gene coding recombinant rhHER2-mAb humanized monoclonal antibody, and its application
2013
The invention discloses a gene coding a recombinant rhHER2-mAb humanized monoclonal antibody, and its application. The gene includes a light chain coding nucleotide sequence represented by SEQ ID NO.1 and a heavy chain coding nucleotide sequence represented by SEQ ID NO.2. A signal peptide, an initiator and a termination codon are respectively designed on the above two sequences; and the designed sequences are respectively cloned into an eukaryotic expression vector and are commonly transferred to a CHO cell to obtain a CHO cell expressing the recombinant rhHER2-mAb humanized monoclonal antibody, and the CHO cell recombinant rhHER2-mAb humanized monoclonal antibody, is fermented. The codon optimization enables the recombinant rhHER2-mAb humanized monoclonal antibody expression level of the transgenic CHO cell to be high; and a fermentation method provided by the invention prolongs the cell growth time, improves the expression level, reduces the production cost and allows high-purity target proteins to be obtained especially after the addition of a supplemented culturing medium.
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