PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS
2018
In order to ensure the ex-situ conservation of a species, knowing the behavior of the seed during storage is necessary, as it is influenced by several factors inherent to the seed and by characteristics of the environment, such as temperature and relative humidity. This study aimed to evaluate the physiological and biochemical alterations on Cedrela fissilis (pink cedar) seeds during storage under different environmental conditions. Seeds were stored at 20oC in desiccators containing salts that established the relative humidity of 34%, 55%, 75%, and 93%. Analysis were performed on fresh seeds (without storage - time zero), which were used as control, and every two months through a period of eight months of storage. At each assessment, the following aspects were quantified: water content; lipid peroxidation; and the activities of catalase and glucose-6-phosphate dehydrogenase. Germination and electrical conductivity tests were analysed. Seed vigor decreased on humidity at 20 °C. It correlated mainly with decrease of glucose-6-phosphate dehydrogenase activity and increase of lipid peroxidation. Keywords : Pink cedar; germination; vigor; enzymes, lipids. Resumo Alteracoes fisiologicas e bioquimicas durante o armazenamento de sementes de Cedrela fissilis Vellozo. Para garantir a conservacao ex-situ de uma especie, e necessario conhecer o comportamento das sementes durante o armazenamento, visto que este e influenciado por varios fatores inerentes a semente e as caracteristicas do ambiente, tais como temperatura e umidade relativa. Este estudo teve como objetivo avaliar as alteracoes fisiologicas e bioquimicas em sementes de Cedrela fissilis (cedro rosa) durante o armazenamento sob diferentes condicoes ambientais. As sementes foram armazenadas a 20oC em dessecadores contendo sais que estabeleceram as umidades relativas do ar de 34%, 55%, 75% e 93%. As analises foram realizadas em sementes frescas (sem armazenamento - tempo zero), usadas como controle, e a cada dois meses durante um periodo de oito meses de armazenamento. Em cada avaliacao, foram quantificados: teor de agua; peroxidacao de lipidos; e as atividades das enzimas catalase e glicose-6-fosfato desidrogenase. Tambem, foi analisada a germinacao e condutividade eletrica das sementes. O vigor das sementes diminuiu em todas as umidades a 20 °C, correlacionando-se, principalmente, com a diminuicao na atividade de glicose-6-fosfato desidrogenase e com o aumento na peroxidacao lipidica. Palavras-chave: Cedro rosa; germinacao; vigor; enzimas, lipidios.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
17
References
2
Citations
NaN
KQI