Determination of lercanidipine in human plasma by LC-MS/MS

Abstract: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed andvalidated for the quantitative determination of lercanidipine in human plasma. After addition of internal standard(amlodipine), plasma was precipitated with acetonitrile and the supernatant was evaporated. The residues weredissolved in 50 % acetonitrile and analyzed by LC-MS/MS. Using MS/MS with multiple reaction monitoring(MRM) mode, lercanindipine were selectively detected without severe interference from human plasma. Thestandard calibration curve for lercanidipine was linear ( r = 0.9994) over the concentration range 0.05-20.0 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of lercanidipine was lowerthan 11.7 % (correlation of variance, CV), and accuracy was between 94.4-114.8 %. This method has beensuccessfully applied to the pharmacokinetic study of lercanidipine in human plasma. Key words: lercanidipine, LC-MS/MS, pharmacokinetic study ★ Corresponding authorPhone : +82-(0)2-2027-6250 Fax : +82-(0)2-2027-6299E-mail : heejoolee0@yahoo.co.kr