Use of an ether-derived 3-hydroxy-4-pyridinone chelator as a new chromogenic reagent in the development of a microfluidic paper-based analytical device for Fe(III) determination in natural waters

Abstract This article reports on the development and validation of a disposable microfluidic paper-based analytical device (μPAD) for on-hand. in-situ, and cheap Fe(III) determination in natural waters complying with World Health Organization guidelines. The developed μPAD used 3-hydroxy-4-pyridinone (3,4-HPO) as a colour reagent due to its considerably lower toxicity than traditionally used iron analytical reagents. It was selected among a group of hydrophilic 3,4-HPO chelators containing ether-derived chains in their structure which were prepared using green methods. The relatively high water solubility of these chelators improved the detection limit and applicability as μPAD reagents. Under optimal conditions, the μPAD is characterised by a quantification range between 0.25 and 2.0 mg/L, a detection limit of 55 μg/L and 15 min of analysis time. The signal stability extends up to 4 h and the device is stable for at least one month. The reagent consumption is below 0.2 mg per analysis and the μPAD method was validated by analysis certified reference materials and by comparison with atomic absorption results (RD