Determination of the size of lipid rafts studied through single-molecule FRET simulations.
2021
Abstract Fluorescence Resonance Energy Transfer (FRET) is a high resolution technique that allows the characterization of spatial and temporal properties of biological structures and mechanisms. In the present work, we developed an in silico single-molecule FRET methodology to study the dynamics of fluorophores inside lipid rafts. We monitored the fluorescence of a single acceptor molecule in the presence of several donor molecules. By looking at the average fluorescence, we selected events with a single acceptor and donor molecules, and we used them to determine the raft size in the range of 5 to 16 nm. We conclude that our method is robust and insensitive to variations in the diffusion coefficient, donor density or selected fluorescence threshold.
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