Estabelecimento in vitro de segmentos caulinares de Araucária (Araucaria angustifolia)

Araucaria angustifolia is a species of high economic value, timber, food and to resin but have problems concerning propagation by seed due to the low viability of propagules. The micropropagation may be an alternative for producing seedlings of good quality. The present study evaluated the in vitro establishment of stem segments of A. angustifolia collected in three different positions and in different shifts in culture media and their dilutions. After 60 days of sowing in plastic trays held excision of stem segments of approximately 2-3 cm in length. The experiment was conducted in a completely randomized design, using a 3 x 4 factorial scheme, in which the levels of factor "A" referred to the different collection positions and levels of the factor "B", the nutrient media (MS full, ½ MS, WPM full and ½ WPM. were six replicates of each explant for treatment. After 60 days of culture in vitro was assessed the percentage of establishment, contamination and callus formation, and the number of shoots per explant and shoot length. Callus formation was lower with the use of MS complete nutrient medium and diluted to 50%. Use the position of explants collected and cultured in basal medium MS had the highest training shoots and greater length. Establishing in vitro Araucaria from explants of seminal origin is most successful when it makes use of suitable nutrient media and observing the position of collection.